《北方园艺》：汉中地区天麻萌发菌遗传多样性和促萌效果分析
《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2024年2 页码: 118-124 栏目: 出版日期: 2024-01-31
Title:Analysis of Genetic Diversity and Germination Promoting Effect of Gastrodia elata Germinating Fungi in Hanzhong
文章编号:1001-0009(2024)02-0118-07
作者:彭浩1; 曹丹1; 司豆豆1; 张升明2; 3; 张怀荣4; 李娜1（1.陕西理工大学 生物科学与工程学院，陕西 汉中 723000；2.陕西春森菌业有限公司，陕西 汉中 723500；3.陕西省西乡县食用菌研究所，陕西 汉中 723500；4.宁强县真菌研究所，陕西 汉中 724400）
Author(s):PENG Hao1; CAO Dan1; SI Doudou1; ZHANG Shengming2; 3; ZHANG Huairong4; LI Na1(1.School of Bioscience and Engineering，Shaanxi University of Technology，Hanzhong，Shaanxi 723000；2.Shaanxi Chunsen Mushroom Industry Co.Ltd.，Hanzhong,Shaanxi 723500；3.Xixiang County Edible Fungi Research Institute，Hanzhong,Shaanxi 723500;4.Ningqiang County Institute of Fungi，Hanzhong,Shaanxi 724400)
关键词:天麻; 萌发菌; 拮抗; ITS; 酯酶同工酶
Keywords:Gastrodia elata; germination fungi; antagonism; ITS; esterase isozyme
分类号:Q 939
DOI:10.11937/bfyy.20232599
文献标志码:A
摘要:以汉中地区9株天麻萌发菌及天麻花粉种子为试材，采用拮抗试验、ITS序列测定、酯酶同工酶技术和种子拌播等方法，研究了9株天麻萌发菌的遗传多样性、亲缘关系和促萌效果，以期为天麻萌发菌的种质资源管理与评价等提供参考依据。结果表明：9株萌发菌根据两两拮抗试验的程度不同分为不显著、显著和极显著3类；根据ITS序列的差异分为Mycena sp.和Heydenia sp. 2类；根据酯酶同工酶酶谱条带在80% 的相似水平上可分为5类；9株萌发菌与天麻种子共生萌发，其中C3菌株菌丝生长速度最高为（0.30±0.01）cm·d-1，C2菌株的萌发率最高为64.66%±2.89%，C2和C3菌株的综合表现最佳。
Abstract:The genetic diversity，genetic relationship and germination promoting effect of 9 Gastrodia elata germinating fungi and their pollen seeds in Hanzhong area were studied by means of antagonistic experiment，ITS sequence determination，esterase isozyme technology and seed mixing，in roder to provide reference for the management and evaluation of germplasm resources of Gastrodia elata germinating fungi.The results showed that 9 germination fungi were classified into 3 types，insignificant，significant and extremely significant according to the degree of antagonism of each pair；according to the difference of ITS sequence，they were classified into Mycena sp.and Heydenia sp.2；according to esterase isozyme.The zymogram bands could be divided into 5 categories at a similar level of 80%；9 germination strains co-germinate with Gastrodia elata seeds，of which the highest growth rate of C3 strain was (0.30±0.01)cm·d-1，and the highest germination rate of C2 strain was 64.66%±2.89%，the comprehensive performance of C2 and C3 strains were the best.
